Sequence Analysis of SOD Gene of Bombyx
mori Nuclear Polyhedrosis
Virus and It’s Expression in E.coli
WANG Wen-Bing,WU Jun,and WU Xiang-Fu
( Shanghai Institute of Biochemistry,the Chinese Academy of
Sciences,Shanghai 200031,China )
WANG Wen-Bing，JI Ping,HE Jia-Lu
( Institute of silkworm,Chinese Academy of Agricultural Sciences,Key
Loboratory of Silkworm Biotechnology,Ministry of Agriculture,Zhenjiang
212018,China )
Abstract Superoxide dismutases scavenge
superoxide radicals and protect cells from oxidative stress.SOD gene of Bombyx
mori nuclear polyhedrosis virus has been cloned by PCR,and is expressed in
E.coli with the activity of SOD being 576.13 u/mL.DNA sequence
analysis shows that SOD gene of BmNPV encodes 151 amino acids.The homology
between BmNPV and AcNPV of the nucleotide sequences of SOD gene is
97.2%,and 56% between BmNPV and human SOD1.
Key Words Superoxide dismutase；BmNPV；sequence analysis；expression
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