Protein and Peptide Identification by
Capillary Zone
Electrophoresis-Tandem Mass Spectrometry
LIU Tao,ZENG Rong,SHAO Xiao-Xia and XIA Qi-Chang
( Shanghai Institute of Biochemistry,the Chinese Academy of
Sciences,Shanghai 200031,China )
Abstract A method for highly sensitive
protein and peptide identification by capillary zone
electrophoresis/electrospray ionization-tandem mass spectrometry
(CZE/ESI/MS/MS) was described. A mixture of peptides Met-enkephalin and
Leu-enkephalin was analyzed,and their amino acid sequences were
identified,respectively,using CZE/MS/MS. Peptide mapping for a tryptic digest
of horse cytochrome c was performed by the same method. The results indicated
that almost all peptides generated by tryptic digestion were identified,and
their sequences were interpreted by their collision-induced (CID) mass spectra.
Applying the uninterpreted CID spectra of the protein digest to searching in
sequence database by the SEQUEST program could identify proteins conveniently
and rapidly. The samples consumed in the CZE/MS/MS experiments were at picomole
level,so this method is quite suitable for quality control of recombinant
proteins and trace amount protein identification in the proteome research.
Key Words Capillary zone electrophoresis/tandem
mass spectrometry；high sensitivity；protein and peptide
identification
Corresponding author:
Tel, 86-21-64374430; Fax, 86-21-64338357; e-mail, [email protected]