Glucocorticoids Modulate G-protein
α-subunit Levels in Rat Hypothalamus
GAO Bo1 *,XIONG Ren-Ping2,YIN
Gui-Shan1 and ZHOU Yuan-Guo2
(1 Department of Biochemistry,Hebei Medical
University,Shijiazhuang 050017,China；
2 Research Institute of Field Surgery,Daping Hospital,Third
Military Medical University,Chongqing 400042,China )
Abstract Glucocorticoids (Gc) affect
neuroendocrine function of hypothalamic-pituitary-adrenal axis
(HPAA),especially of the hypothalamus. Synthesis and secretion of neuronal
hormones of hypothalamus are associated with G-protein-linked signalling
system. Here,the regulation by Gc of the levels of several G-protein α-subunits
in rat hypothalamus was studied with Western blot method. Results showed that
Gc downregulated Gαs1,Gαs2,Gαi and Gαq with different magnitudes,being
14.37,15.66,14.61 and 12.37 per cent,respectively. However,Gc did not affect
Gαo expression. Our results provided important information for understanding
how Gc alters neuroendocrine function through G-protein-linked signalling
system.
Key Words Hypothalamus；G-protein；glucocorticoids
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