Syp Y279,Y304 Can Mediate the Binding of
Bcr-Abl
to Grb2 and Other Proteins
ZHU Kui1, LIU Xiao-Ping2, YE Fang-Yun1
(1 Clinic Lab./Research Center,Changhai Hospital、Tumor Immunology and Gene
Therapy Center,
Institute of Eastern Hepatobiliary Surgery,Second Military Medical
University,Shanghai
200433,China )
ZENG Grant
( Department of Biochemistry,John P.Robert Research Institute,
The University of Western Ontario,100 Perth Drive,London N6A 5K8,Canada
)
Abstract In this study we obtained 3
mutants of Syp cDNA Y279F,Y304F and Y546F by using the method of site-directed
mutation in vitro.We then inserted them into the pXM mammalian
expression vector,and transferred the plasmids into K562 cells.Western Blot has
found WT/ 3MT Syp are expressed in the K562 cells.Immunoprecipitation and
Immunoblot have found that WT,Y279F,Y304F and Y546F Syp can bind directly to
the Bcr-Abl in vivo.However,we found that mutation of Syp Y279F can
block the binding of Grb2 SH2 to Syp；mutation of Syp Y304F can
block the binding of Shc SH2 to Syp in vitro.As a adaptor,Syp can
mediate binding of Bcr-Abl to Shc and Grb2.Grb2 bind to Syp pY279,Shc bind to
Syp pY304 in vitro.
Key Words Bcr-Abl tyrosine kinase；Syp protein；signal transduction
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