Potential GM-CSF Antagonists Selected
from a Peptide Phage Display Library
TANG Wei-Gang,GAO Xiang,CHE Fa-Yun,GAN Ren-Bao and WANG
Ke-Yi
( Shanghai Institute of Biochemistry,the Chinese Academy of
Sciences,Shanghai 200031,China )
Abstract Peptide phage display
libraries have been successfully applied in areas of mapping antibody
epitope,finding ligands for enzymes,receptors,and many other molecules.But it
has been demonstrated to be very difficult to select cytokine-binders from peptide
phage display libraries probably because cytokine is not so sticky as antibody
that there are rare chances of capturing peptide phages during biopanning.A
pVIII-based peptide phage display library was panned with the cytokine GM-CSF
and some GM-CSF binding clones were selected based on high throughput screening
(HTS) method and confirmed by ELISA and micropanning assays.These
cytokine-binders may be utilized in affinity chromatography in cytokine
downstream processing and even act as potential antagonists of GM-CSF if their
affinity are further improved through secondary library strategy.
Key Words Phage display；peptide；GM-CSF；drug target
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