Activity of Lipase in Reversed Micelles
LU Hong* and FAN Pei-Chang
( Department of Biology,East China Normal University,Shanghai 200062,China )
Abstract The enzyme activity of lipase
from Candida rugosa in AOT isooctane reversed micelles was
investigated. Effect of pH,temperature,substrate concentration,waterpool (R
value),choice of organic solvent,surfactant concentration on the activity of
lipase were studied in detail. Results indicate that the enzyme in reversed
micelles follows the Michaelis-Menten dynamics：temperature optimum=32.5 ℃,pH
optimum=7.0,and maximum activity is reached at the concentration of olive oil
of 40%. The enzyme activity was dependent on R (molar ratio of water to
surfactant) and maximum activity was obtained at R=11.
Key Words Lipase；reversed micelles；enzyme reaction
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