Preparation,Characteristics and Toxicity
of Ricin-containing
Galactosyl Ceramide Liposomes
ZHANG Jin and QIN De-An
( Department of Biology,East China Normal University,Shanghai 200062,China
)
CHENG Bai-Xian,RU Xiao-Qin,LI Hao,LI Ling and YANG Hui-Pin
( Gan Quan Hospital,Shanghai 200070,China )
Abstract Ricin-containing galactosyl
ceramide liposomes (ricin-GCLs) lyophilized product was made by the
dehydration-rehydration vesicles(DRV) method and its toxicity to human
heptocarcinoma cell line 7721 and to mice was investigated. The results
indicated that the maximum encapsulation efficiency of ricin was 74.1%. The
distribution of ricin-GCLs’ sizes accords with intravenous injection standard
and changes little in half a year at 4 ℃. In the cytotoxicity assay,the ratio
of ricin-GCLs’ to free ricin’s IC50 was 1.00∶3.89,which indicated
that the ricin entrapped in GCLs was more effective than the free one in
killing human heptocarcinoma cell line 7721. In vivo,the mice’s LD50
of ricin-GCLs ((41.8±9.33) μg/kg)is more than that of free ricin ((30.6±8.49)
μg/kg) by 36.29%. So,ricin entrapped in GCLs promotes its cytotoxicity and
reduces its toxic side-effect.
Key Words Ricin；galactosyl ceramide
liposomecytotoxicity
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