Separation and Characterization of
Oligodeoxynucleotides by FPLC
TANG Hui-Wei,JIN You-Xin
( State Key Laboratory of Molecular Biology,Shanghai Institute of
Biochemistry,the Chinese Academy of Sciences,Shanghai 200031,China )
TANG Hui-Wei ZHU De-Xu
( Department of Biochemistry,Nanjing University,Nanjing 210008,China
)
Abstract The purity of the antisense
drugs synthesized by DNA synthesizer should be determined effectively. At pH
12,the oligodeoxynucleotides (ODNs) ranged from 19 bases to 21 bases can be
separated successfully on the anion exchange column MONO-Q by elution with a
NaCl gradient. The experiments proved that the fast protein liquid
chromatography (FPLC) was a useful tool for isolation and identification of the
antisense drugs.
Key Words FPLC；oligodeoxynucleotide；antisense drug
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