Analysis of Phylogenetic Relationships
Among Twelve Yeast Species
ZHANG Ping-Wu,LUO Ze-Wei,CHEN Li,HUO Ke-Ke and LI Yu-Yang
( Institute of Genetics,State Key Laboratory of Genetics,Fudan
University,Shanghai 200433,China )
Abstract Partial sequences of 25 S
ribosomal DNA for nine Candida yeast and one Kluyveromyces yeast strains were
cloned and determined. We compared them with two published 25 S rDNA sequences
of Saccharomyces cerevisiae and Candida albicans. An
evolutionary tree of the twelve species was inferred from about 370 sites of 5′
end of 25 S ribosomal DNA using the methods of neighbor-joining and bootstrap .
The molecular data indicate a very close affinity between Candida kefyr
CBS834 and Kluyveromyces cicerisporus CBS4857.
Key Words Candida yeast；ribosomal RNA；DNA sequence；phylogenetic tree
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