Cloning of a Gene in Rat
Brain Induced to Be Expressed
Differently by AVP4-8 with Differential Display PCR
XIONG Ying, WANG Ying, LIU Xiao-Long and DU Yu-Cang*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract As a metabolite of
arginine-vasopressin, AVP4―8 has been shown to have potent
memory-enhancing activity and induce a series of physiological and biochemical
events in rat brain. The technique known as differential display polymerase
chain reaction(DD-PCR)was applied to explore genes induced to be expressed
differ-ently by AVP4―8 in rat hippocampus. Nine different primer
pairs were used to perform DD-PCR after reverse transcription, and more than
ten differential display fragments were observed. The most remarkable different
fragment, dd1, was further cloned and sequenced. Homology searching in Genebank
showed that dd1 may be a new gene. Moreover, the results from Northern blot
confirmed that dd1 is indeed a gene induced by AVP4―8.
Key Words cloning； AVP4-8； DD-PCR； rat brain
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