Telomere Shortening of
MCF-7 Cells Caused by Antisense Telomerase cDNA
ZHANG Xiao-Wei, LIAO Hai-Ni and TONG Tan-Jun*
( Department of Biochemistry and Molecular Biology, Beijing Medical
University, Beijing 100083, China )
Abstract pAdE1CMVITREXneo
is a novel adenovirus vector which can integrate foreign genes into genome of
host cells. The cDNA of human telomerase RNA was integrated inversely into the
vector via antisense oligonucleotide technique. Antisense recombinant virus
(vAdT-AAV) was obtained by cotransfecting pAdT and pBHG11 into 293
cells. The telomere length of breast cancer MCF-7 cells was found shortened
after the transfection by vAdT-AAV.
Key Words telomerase； antisense cDNA； telomere
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