Application of Free-flow Electrophoresis
to the Separation of Murine Lymphocytes
LIU Tao, SHEN Xin, WU Gao-De and XIA Qi-Chang
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
WANG Sheng-Nian, WU Zhi-Jiang and HU Wei
( Shanghai Institute of Cell Biology, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Free-flow electrophoresis
offers not only the all-in-solution separation process but also very gentle
separation conditions, and it can be used for continuous separation and
preparation, so it has been widely applied in the fields of biochemistry and
cell biology. Using this technique to separate insoluble particles such as
cells, both high separation efficiency and high activity and viability could be
obtained. Murine lymphocytes were separated in low ionic strength
triethanolamine buffer by free-flow electrophoresis, the cell fractions were
detected and characterized by means of UV spectrometry, immune fluorescence
labeling and flow cytometry. Results indicated that T and B lymphocytes were
well separated with high viability.
Key Words free-flow electrophoresis； triethanolamine buffer； separation； lymphocyte； viability
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