Preparation and Structural Analysis of
Sulfated Derivatives of Lentinan
WANG Shun-Chun and FANG Ji-Nian*
( Shanghai Institute of Materia Medica, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract A series of sulfated
derivatives of lentinan were prepared by the modified Wolfrom method. Sulfates
content analysis indicated that the proportion of sulfate substitution depended
on the reaction time and the ratio of chlorsulfonic acid and pyridine in the
ester reagent. Methylation analysis by Needs method and Hakomori method was
proved not to be suitable for the structural analysis of sulfated lentinan. 13C-NMR
data showed that sulfates were located on the carbon-6 in all sulfated
derivatives of lentinan showing the higher reactivity of the hydroxyls on C-6
position than those at other position.
Key Words Lentinan； sulfation； methylation analysis； 13C-NMR
Corresponding author:
Tel, 86-21-64311833; Fax, 86-21-64370269