Expression of E-selectin
in Endothelial Cells Effects of Wild Type p53 Gene
LIN Yong, LI Jian1, GAO Cun-Ji2 and
WANG Zhong2
( Department of Pharmacology, Capital University of Medical Sciences,
Beijing 100054, China； 1Department of Biochemistry, Institute of Gerontology, Ministry of
Health, Beijing 100005,
China； 2Institute of Basic Medical Sciences, Chinese Academy of Medical
Sciences, Beijing 100005,
China )
Abstract To
investigate expression of E-selectin in endothelial cells(EC)and effect of wild
type p53 gene transduction on its expression, flow cytometry and
RT-PCR were employed to measure the E-selectin protein and mRNA, respectively.
The effects of TNFα stimulation and p53 gene transduction on the
expression of E-selectin was studied. Flow cytometric analysis revealed that
there was no E-selectin expression on the surface of resting EC, although its
mRNA was detectable by RT-PCR. TNFα(10―1 000 ku/L)induced E-selectin expression
on the surface of EC in a concentration-dependent manner. The level of
E-selectin mRNA was also increased with the increasing concentrations of TNFα.
The expression of E-selectin protein was notable after 2 h of TNFα stimulation
and reached a peak at 4―6 h, then rapidly declined to around the basic level by
12 h. Transduction of p53 gene inhibited TNFα-induced EC expression of
E-selectin. The E-selectin positive cell proportion was reduced
from(21.31±1.06)% to(11.83±0.98)%, (n = 3, P < 0.001). The
E-selectin mRNA level was also reduced correspondingly. In resting EC, neither
E-selectin nor its mRNA level was affected by the transduction of p53
gene. The results indicated that p53 gene inhibited TNFα-induced
expression of E-selectin on the surface of EC, at least partially, by reducing
its mRNA level.
Key Words endothelial cell(EC)； E-selectin； TNFα； p53 gene； gene transduction
Received： April 8, 1999 Accepted： June 8, 1999
Corresponding author： Tel, 86-10-63051453； e-mail, [email protected]