Analysis of 5′ Upstream
Regulatory Sequences of Lignin Peroxidase(LIP) Genes of Phanerochaete
chrysosporium
FENG Hong and ZHANG Yi-Zheng
( Molecular Biology Laboratory, College of Life Science, Sichuan
University, Chengdu 610064, China )
Abstract Phanerochaete
chrysosporium yields exocellular isozymes of lignin peroxidases and Mn
peroxidases which are able to degrade lignin. In order to investigate the
regulatory mechanism of LIP genes at transcriptional level, 6 DNA
fragments subcloned from 5′ upstream sequences of LIP genes (GLG3
and GLG6) were obtained, then gel mobility shift assay was carried out
to screen DNA fragment(s) with ability to be bound specifically by proteins
isolated from P.chrysosporium mycelia. The results showed that a 670
bp DNA fragment localized upstream of a LIP gene GLG6 was
capable of being bound specifically by protein component, and analysis of its
nucleotide sequence indicated that the DNA fragment contains the putative
sequence characteristic of protein binding site. This result suggests that
protein(s) in this fungus interact with the putative cis-element(s) in
the 5′ upstream sequence of LIP genes, regulating the expression of LIP
genes.
Key Words Phanerochaete chrysosporium； gel mobility shift assay； DNA-bonud protein； cis-regulatory element
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