Cloning and Expression
of Human Manganese-superoxide Dismutase cDNA
SHI Hui-Juan1，HE Hua-Jun，WEI Dong-Zhi and YUAN
Qin-Sheng
( Research Institute of Biochemistry，State Key Laboratory of
Bioreactor Engineering，East China University of Science and
Technology，Shanghai 200237，China )
CHEN Zhe-Yu，LI Ming-Feng and WU Xiang-Fu
( 1Shanghai Institute of Biochemistry，the Chinese Academy of
Sciences，Shanghai 200031，China )
Abstract The cDNA
encoding human manganese-superoxide dismutase was amplified from the human
liver cell (L02) total RNA by RT-PCR and sequenced.The human Mn-SOD
cDNA was ligated into expression vector pET-24a(+) under T7 promoter.After 5 h
induction with 1 mmol/L IPTG，800μmol/L Mn2+，human Mn-SOD was
highly expressed in E.coli BL21(DE3).The protein product was up to 50%
of the bacteria total protein in soluble form，and had specific SOD activity.
Key Words manganese-superoxide dismutase；expression vector；gene cloning；gene expression；E.coli
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