Gene Expression Profiles
of Human Fetal Nasopharyngeal Tissue
HE Zhi-Wei, XU Liang-Guo, XIE Lu, ZHANG Ling, LAN Ke, REN
Cai-Ping and YAO Kai-Tai
( Key Laboratory of Carcinogenesis of Chinese Ministry of Public Health of
Cancer Research Institute of Hunan Medical University, Changsha 410078,
China )
Abstract To study
differentially expressed genes in nasopharynx tissues of embryo during
development, and to observe the gene changes, total RNAs were respectively
extracted from the nasopharynx tissue which came from fetuses of 5, 6, 7, and 8
month, Probes were yielded by reverse transcription and were used to hybridize
with the AtlasTM human cDNA expression arrays. The results showed
that the genes expression profile were distinctly different and different
expression levels were found in the same gene during development. Results
indicated that gene expression pattern had a character of time-dependence, such
as early growth response protein 1； and the cDNA expression array
provided a powerful method for studying gene expression profile in a large
range of genes.
Key Words cDNA expression array； embryo development； nasopharynx tissue； gene expression profile
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