Adsorption on Cellulose
of the Schizophyllum commune Cellobiose
Dehydrogenase
FANG Jing and GAO Pei-Ji
( The State Key Lab of Microbial Technology, Shandong University, Jinan 250100,
China )
Abstract Equilibrium
of adsorption of cellobiose dehydrogenase (CDH) of Schizophyllum commune
on cotton cellulose, microcrystalline cellulose, and phosphate-swollen
cellulose was reached within 4 min. The binding isotherms of CDH adsorbed on
the above substrates were a straight line in Scatchard plot analysis,
indicating that the binding fitted to the one-binding-site model. It was found
that CDH adsorption onto microcrystalline cellulose was strongly affected by
temperature, pH, NaCl, and ethylene glycol, respectively.
Key Words cellobiose dehydrogenase； cellulose binding； cellulose； cellulase
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