Fusion Expression and
Antibody Preparation of the Human Transcription Factor hB1F
CAI Yan-Ning, WANG Yuan*
(Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China)
Abstract Human B1F
was cloned from a human liver cDNA library by yeast one-hybrid screening and
characterized. It is a transcription factor which belongs to nuclear receptor
superfamily. The cDNA segment （450―930） of human liver
transcription factor （hB1F） was cloned into the
pGEX-3X expression vector and was expressed in E.coli. The purified
GST-fused hB1F, named GST-CS, were used to immunize mice to get polyclonal
antibodies to hB1F. The antiserum showed specificity to hB1F after the
purification by GST-Sepharose 4B. It can be used in further investigations of
the structure and function of hB1F.
Key words transcription factor； nuclear receptor； fusion expression； antibody； affinity chromatography
Recevied: July 1,
1999 Accepted: August 20, 1999
* Corresponding author: Tel, 86-21-64374430; Fax, 86-21-64338357; e-mail, [email protected]