Exogenous p21WAF1 Transfection
Affects Apoptosis in Human Fibroblasts
HUANG Ying, YANG Long, ZHANG Zong-Yu, TONG Tan-Jun
( Department of Biochemistry and Molecular Biology, Beijing Medical
University, Beijing 100083, China )
Abstract pDOR-p21sense
and pDOR-p21 antisense retroviral expression vectors were constructed
and successfully transfected by Lipofectin into normal human diploid
fibroblasts(2BS line), resulting in 2BS-p21s and 2BS-p21a
cell lines, respectively. Southern blot analysis verified that the exogenous p21WAF1 cDNA were integrated into
genomic DNA. Compared with the cells transfected with pDOR-neo empty
vector, p21WAF1 mRNA expression increased in 2BS-p21s
cells, which were less sensitive to apoptosis induced by NaBu, and showed
higher cell viability, delayed appearance of DNA ladder, and less area of
apoptosis peak. On the other hand, p21WAF1 mRNA expression decreased in
2BS-p21a cells, which were more sensitive to apoptosis induced by
NaBu. These results indicated that the expression amount of p21WAF1 in 2BS cells was negatively
related with its susceptibility to apoptosis induced by NaBu.
Key words p21WAF1； cell apoptosis； gene transfection； sodium butyrate
Received: May 22,
1999 Accepted: July 6, 1999
* Corresponding author: Tel, 86-10-62091454; Fax, 86-10-62015582; e-mail, [email protected]