Purification and
Functional Characterization of a Shark Cartilage Factor Inhibitory to
Angiogenesis
SHEN Xian-Rong2*, JI Dong-Mei1, JIA Fu-Xing2, DENG Xin-Xian1, SUN Jia-Hong2, HU Xing-Ran1, REN Da-Ming1
( 1State Key Lab of Genetic
Engineering, Institute of Genetics, Fudan University； 2Naval Medical Research
Institute, Shanghai 200433, China)
Abstract SCAIF-I,
an inhibitor of angiogenesis from shark cartilage was purified to homogeneity.
The 4 mol/L guanidinium chloride extract of shark cartilage was fractionally
precipitated with 35%―65% acetone, then purified by Resource Q ion exchange
chromatography, Sephacryl S-300 gel filtration, and reverse-phase high
performance liquid chromatography. The pure inhibitor was homogeneous as a
single band on a silver-stained 15% sodium dodecyl sulfate-polyacrylamide gel.
SCAIF-I had an molecular weight of 18 kD. It specifically inhibited
proliferation of endothelial cells, and strongly blocked endothelial cell movement
and angiogenesis in the chorioallantoic membrane of chick embryos. Systemic
administration of SCAIF-I at the dose of 5 mg/kg*d suppressed 87.93% of the
growth of Lewis lung carcinoma implanted in C57BL/6 mice.
Key Words shark； cartilage； angiogenesis； endothelial cell； tumor therapy
Received: July 22,
1999 Accepted: september 1, 1999
* Corresponding author: Tel, 86-21-65642506; Fax, 86-21-65648376; e-mail, [email protected]