An NF-IL6 3′UTR
RNA-specific Binding Protein in E. coli：Purification and Partial Protein
Sequencing
QING Guo-Liang, LIU Hu-Dan, LIU Ding-Gan*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract A protein
was found in E.coli which can specifically bind to NF-IL6 mRNA 3′UTR. After a
series of purification steps, the RNA-specific binding protein was directly
sequenced on the Porton LF3200 Protein Sequencer. A sequence of 10 amino acids
of the purified NF-IL6 3′UTR binding protein was obtained, namely,
Ala-Thr-Arg-Ile-Glu-Phe-His-Gly-Cyss(?)-Gly. A BLAST search with the 10 amino
acids against NCBI database failed to identify any protein with identical
sequence. The significance of identifying an eukaryotic mRNA binding protein in
E.coli is discussed.
Key Words NF-IL6 3′UTR； RNA binding protein； RNA affinity
chromatography；
Protein sequencing
Received: July 9,
1999 Accepted: September 8, 1999
Supported by a grant of the State High-Tech R & D Project(863 Project)
* Corresponding author: Tel, 86-21-64374430-340; Fax, 86-21-64338357; e-mail, [email protected]