Purification
and Characterization of a Novel Anticoagulation Factor (ACF II) from the Venom
of Agkistrodon acutus
XU Xiao-Long, LIU Qing-Liang*
( Department of Chemistry, University of Science and Technology of China,
Hefei 230026, China )
Abstract A novel
anticoagulation factor (ACF II) was first isolated from the venom of Agkistrodon
acutus by DEAE-Sephadex A-50, Sephadex G-75 and CM-Sephadex C-50 column
chromatography. The purified ACF II showed a single band in PAGE, SDS-PAGE and
IEF-PAGE, respectively. ACF II consisted of two peptide chains linked together
by disulfide bond(s) and the molecular weights of the two peptide chains were
about 14.6 kD as determined by SDS-PAGE. The isoelectric point of ACF II was
7.0. ACF II possessed distinct anticoagulant activity and its minimum
concentration to prolong plasma prothrombin time in vitro was 0.4 mg/L.
No thrombin-like activity, fibrinolytic activity, phospholipase A2
activity, haemorrhagicactivity, or lethal activity was detected in ACF II,
which may be useful as an effective venom-based anticoagulant.
Key words Agkistrodon acutus；anticoagulation factor；purification.
*Corresponding author：Tel, 86-551-3603214；Fax,86-551-3631760；
e-mail, [email protected]