Expression of the
Recombinant Hepatitis B Virus Surface Antigen Carrying PreS Epitopes in Pichia
pastoris
YANG Jian-Ying, HUI Jing-Yi, LI Guang-Di, WANG Yuan
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
YUAN Han-Ying, LI Yu-Yang
( Institute of Genetics, Fudan University, Shanghai 200433, China
)
Abstract Many
studies have suggested that hepatitis B surface antigen(HBsAg) including PreS
sequences could be an ideal candidate for highly effective hepatitis B virus
vaccine. Modified surface antigens S1S, SS1 and S2S which carried PreS
epitopes, were expressed in Pichia pastoris. The characterization of
antigenicity and particle assembly demonstrated that the expression products
could be assembled into particles which presented S, PreS1 or PreS2
antigenicity, respectively. The expression was more efficient than that in Saccharomyces
cerevisiae.
Key words hepatitis B virus surface antigen； PreS； Pichia pastoris； expression； characterization
Corresponding author： Tel, 86-21-64374430； Fax, 86-21-64338357； e-mail, [email protected]