Isolation and
Identification of cDNA Sequences Differentially Expressed in Laryngeal
Carcinoma
LI You-Jun, TIAN Fang, CHEN Zhu-Chu, GUAN Yong-Jun, HE
Chun-Mei, YANG Xin-Ming, XIE Ding-Hua
( Cancer Research Institute of Hunan Medical University, Changsha 410078,
China )
Abstract The
isolation of the genes related to laryngeal carcinoma(LC) is necessary for
revealing mechanisms of carcinogenesis and genetic predisposition to LC. The
mRNA differential display method was used to compare and analyze mRNAs prepared
from two adult laryngeal carcinoma tissues and paired tumor-adjacent normal
tissues. A total of twenty-two differential display experiments was performed
and thirty-five cDNA fragments differentially expressed in normal or malignant
laryngeal epithelial tissues were identified. Differential expression of six of
these thirty-five cDNA fragmens was confirmed by reverse northern dot blot.
Subsequent cloning of six differentially expressed cDNA fragments and
sequencing and BLASTn analysis resulted in the identification of twelve
distinct cDNA sequences. Four of these were shown to be novel gene sequences
that have not been reported. Eight of the remaining cDNA sequences showed
sequence homology to those previously reported. The differential expression of
these twelve cDNA sequences in the carcinoma or normal tissue of the larynx
were confirmed by fixing the twelve cDNA sequences on the membrane, followed by
the hybridization with the total cDNA probes from laryngeal carcinoma or normal
tumor-adjacent tissues and by differential RT-PCR. These results suggest that
these cDNA sequences might be involved in carcinogenesis of laryngeal
carcinoma.
Key words laryngeal carcinoma； mRNA differential display； differential expression
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