Application of the
Fragments and Structure Analysis of Matrix-assisted Laser Desorption/Ionization
Time-of-flight Mass Spectrometry in Peptide Sequencing
DENG Hui-Min, LAI Zhi-Hui, LI Jun, ZHA Qing-Min, ZHAO
Shan-Kai
( Instrumental Analysis & Research Center, Zhongshan University,
Guangzhou 510275, China )
Abstract The
peptides of angiotensin II and fragment 18―39 of adrenocorticotropic hormone
were determined by MALDI-TOF-MS-FAST. Main amino acid compositions and partial
sequences of the peptides have been analyzed by the mass data of fragment ion
peaks in the FAST spectra. The primary structures of these two peptides were
identified by searching the protein databases. The research results showed that
FAST is a powerful tool for determining the primary structure of peptides
because of its high sensitivity, quickness and accuracy.
Key words MALDI-TOF-MS-FAST； sequencing； angiotensin II； adrenocorticotropic hormone
fragment 18―39
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