The Primary Study on
Expression and Function of D-Type Cyclins in Nasopharyngeal Carcinoma Cell
Lines
ZHAO Xiao-Rong, GU Huan-Hua, WENG Xin-Xian, YI Wei, DENG
Xi-Yun, CAO Ya
( Cancer Research Institute of Hunan Medical University, Changsha 410078,
China )
Abstract Previous
work of the authors established the protein expression spectra of D-type
cyclins, and found that there were differential expression in nasopharyngeal
carcinoma (NPC) biopsies. Using Western Blotting, aberrant overexpression of
the cyclinD1 protein in NPC cell lines is reported. CyclinD2 and cyclinD3,the
other members of D-type cyclins, were also detected in NPC cell lines. A
comparison of the expression patterns in the cell lines, positive or negative
in the latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus, revealed
that the expression of D-Type cyclin proteins might be increased by EBV-LMP1.
The abundance of these proteins showed characteristic variation consistent with
a cell- cycle-dependent oscillation and the peak levels expressed in G1 as
analyzed by double-parameter flow cytometry. These data suggest that cyclinD1
is essential for cell cycle progression in G1 and may play a role in NPC carcinogenesis.
Key words D-Type cyclin； nasopharyngeal carcinoma cell
line；
Epstein-Barr virus； latent membrane protein 1
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