Transcription and
Translation of Sendai Virus Hemagglutinin-neuraminidase (HN) in Mammalian Cells
HU Jian-Hong, QI Bing, NAKANISHI Mahito1, QI
Yi-Peng*
( Institute of Virology, Wuhan University, Wuhan 430072, China;
1Institute for Microbial Diseases, Osaka University, Osaka
565, Japan )
Abstract Sendai virus
hemagglutinin-neuraminidase was expressed in COS-7 cells. LLCMK2
cells were transfected with expression plasmids harboring HN, namely pJH3, pJH4 or
pJH7,and were passaged conti-nuously under the presence of hygromycin or
puromycin to get antibiotic-resistant clones, which had HN gene integrated into genomes.
S1 nuclease assay indicated that large quantity of HN mRNA was transcribed but
indirect-immunofluorescence and immunoprecipitation demonstrated that there was
little HN protein expressed on cell surface and inside cells, in contrast with
the Sendai virus persistent-infected LLCMK2 cells that expressed
largely HN protein. Therefore, not every transcription resulted in translation.
It also suggests that some virus factors may positively regulate transcription
and translation of HN mRNA.
Key words Sendai virus； haemagglutinin-neuraminidase； transcription； translation
Received: November
17, 1999 Accepted: December 23, 1999
* Corresponding author: Tel, 86-27-87682938; e-mail, [email protected]