Expression of Smad4
Gene in Pichia pastoris and
Identification of the Protein
LIU Yi-Ping1, YANG Xiao, XU Xiao-Ling2,
DENG Ji-Xian, CHENG Xuan, HUANG Cui-Fen*
( Institute of Biotechnology, Academy of Military Medical Sciences, Beijing
100071, China; 1Guangzhou Institute of Military Medical
Sciences, Guangzhou 510507, China; 2National
Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20892, USA
)
Abstract Smad4 is a novel
tumor suppressor gene which is mutated or deleted in about 50% of pancreatic
carcinoma and 30% of colon cancer. SMAD4 was expressed in Pichia pastoris and was
characterized. The molecular weight of the expression product was shown to be
about 67 kD, and 13 amino acids in N terminal were determined and were
identical with the putative sequence from Smad4 cDNA, and it was able to
specifically react with the antibody against SMAD4.
Key words Smad4； Pichia
pastoris； yeast expression
Received:
October 12, 1999 Accepted: December 22, 1999
* Corresponding author: Tel, 86-10-66930315; Fax,
86-10-68186320; e-mail, [email protected]