Infectious Bursal
Disease Virus Structural Protein VP2 Expressed by a Baculovirus Recombinant in Bombyx mori
SONG Kun-Hua1,2, JIN Yong-Feng2, HUANG
Yao-Wei1, ZHANG Yao-Zhou2, YU Lian1*
( 1Institute of Animal Immunology; 2Institute of
Biochemistry, Zhejiang University, Hangzhou 310029, China )
Abstract VP2 cDNA gene of the infectious
bursal disease virus HZ96 strain, encoding a major host-protective antigen, was
cloned into baculovirus transfer vector pBacPAK8, resulting in a recombinant
transfer vector pBacPAK-VP2. The vector pBacPAK-VP2 and linearized DNA of
modified baculovirus Bm-BacPAK6 were co-transfected into the cultured Bombyx mori (Bm) N cells,
in which homologous recombination occurred. Then, baculovirus recombinants were
screened out. The Bm cells and Bm larvae were infected with the baculovirus
recombinant that can expresse VP2, and Bm N cells and haemolymph of Bm larvae
were collected for assays. The results of ELISA and Western immunoblotting
assays demonstrated that VP2 was expressed in the cultured Bm cells and the Bm
larvae.
Key words infectious bursal disease
virus； VP2； recombinant Bombyx mori baculovirus
Received:
December 1, 1999 Accepted: december 28, 1999
This work was supported by Zhejiang Commission of Science &
Technology, No. 961102168
* Corresponding author: Tel, 86-571-7054001; Fax:
86-571-7054007; e-mail, [email protected]