High Expression of Human
Persephin in Insect Cells
CHEN Zhe-Yu1,2, HE Zhi-Yong2, HE Cheng1,
LU Chang-Lin1, WU Xiang-Fu2*
(1Department of Neurobiology, the Second Military Medical
University, Shanghai 200433, China; 2Shanghai
Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai
200031, China )
Abstract The human
persephin (PSP) was expressed in Tn-5B1-4 insect cells using the Bac to Bac
baculovirus expression system. The expressed product amounted for 20% of total
cellular soluble proteins. The expressed product was purified by Ni2+
affinity chromatography and the activity assays showed that it could
significantly prolong the survival of spinal cord neurons.
Key words recombinant PSP； baculovirus expression system (BES)； spinal cord neuron
Received:
November 22, 1999 Accepted: december 27, 1999
This work was supported by State Key Program of Basic
Research(973)
* Corresponding author: Tel, 86-21-64374430-292; Fax,
86-21-64338357; e-mail, [email protected]