Expression of Human
Epiregulin in E. coli
XI Quan-Sheng, QIAN Xin-Guo, ZHOU Qing-Wei, PAN Wei, LI
Zai-Ping, GAN Ren-Bao*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Human
epiregulin cDNA was amplified from the lung cancer cell line A549 using RT-PCR.
After adding 6 His codon to its 3′end, it was cloned into a high efficient
secretive Escherichia
coli
system with alkaline phosphatase promoter(phoA promoter)constructed in our
lab and induced for expression. The product was purified one-step by Ni-NTA
column. Amino acid sequence analysis revealed the identity of our product with
that previously reported. The product showed strong proliferative effect on
fibroblast cell line Balb/c3T3 and growth inhibitory effect on epithelial
carcinoma cell line A431.
Key words epiregulin； expression； purification； growth promotion； growth inhibition
Received:
November 12, 1999 Accepted: December 24, 1999
* Corresponding author: Tel, 86-21-64374430-318; Fax,
86-21-64338357; e-mail, [email protected]