Analysis and Molecular
Cloning of Differentially Expressing Genes in Nasopharyngeal Carcinoma
YU Ying, ZHANG Bi-Cheng, XIE Yi, CAO Li, ZHOU Ming, ZHAN
Feng-Huang, LI Gui-Yuan*
( Cancer Research Institute, Hunan Medical University, Changsha 410078,
China )
Abstract In order to
further examine expression of cDNA fragments isolated by cDNA representational
difference analysis(cDNA RDA) in nasopharyngeal carcinoma(NPC) biopsies and to
clone those deregulated genes associated with NPC, RT-PCR and Northern blot
were used to identify the differentially expressed cDNA fragments in NPC
biopsies and confirm the transcript length of those genes, then a full-length
cDNA sequences was cloned and its product was analyzed by bioinformatics. The
results showed that AF091521, AF091520, AF152605 and
AF091517 cDNA sequences had distinct expression difference between
primary cultural normal nasopharyngeal epithelial cell and NPC biopsies, and AF091521,
AF091517 genes all had two transcripts whose sizes were 1.5, 2.3 and
1.1, 1.4 kb respectively, while AF091520 and AF152605 gene
expressed one transcript only, respectively, whose sizes were 1.6 and 2.2 kb.
An AF091517 EST gene, named as NAG11, (GenBank accession
number：
AF170307) was isolated by sequencing one EST clone, which encoded a
transmembrane protein of 88 amino acid including three protein ATP-binding
regions, two protein kinase C phosphorylation sites and two N-myristoylation
sites. So it is further demonstrated that NPC is a disease with multiple gene
alterations；
NAG11 gene is a candidate of
putative tumor suppressor genes associated with NPC, whose down-expression may
be involved in the development of NPC； and NAG11 gene
product may play a role in the transmembrane transport of ATP.
Key words nasopharyngeal neoplasms； gene expression； tumor suppressor gene； cloning
*Corresponding author： Tel, 86-731-4805446； Fax, 86-731-4805446； e-mail, [email protected]