Inhibitory Effects of
Antioxidants on NF-ΚB Activation in Bovine Carotid Artery Endothelial Cells
ZHAO Feng1, MUROTA Sei-itsu2, MORITA
Ikuo2, YAO Xin-Sheng1*
( 1Department of Natural Products Chemistry, Shenyang
Pharmaceutical University, Shenyang 110015, China； 2Department of Cellular Physiological
Chemistry, Graduate School of Tokyo Medical and Dental University, Tokyo 113-8549, Japan )
Abstract NF-ΚB is
one of important transcription factor, which plays an important role in many
immune and inflammatory responses. A large variety of stimulus can induce the
degradation of IΚB-α, an inhibitor of NF-ΚB, leading to the activation and
translocation of NF-ΚB into nuclei. In the present study, the effects of
antioxidants on TNF-α-induced NF-ΚB activation were tested by using cultured
bovine carotid artery endothelial cells. The degradation of IΚB-α were checked
by Western blot, and the nuclear translocation of NF-ΚB were tested by
immunofluorescence. Our results showed that pyrrolidine dithio-carbamate (PDTC)
completely inhibited IΚB-α degradation and nuclear translocation of NF-ΚB
induced by TNF-α, and other antioxidants also exhibited inhibitory activities
on TNF-α-induced IΚB-α degradation.
Key words oxygen stress； NF-ΚB； IΚB-α； antioxidants； PDTC
*Corresponding author： Tel, 86-24-23843711-3389； Fax, 86-24-23993994； e-mail, [email protected]