Phosphatidylinositol(PI)-3
Kinase Contributes to the Activation of Stat3 in M1 Leukemia Cells Induced by
IL-6
ZHANG Ji-Yan*, SHEN Bei-Fen
( Department of Immunology, Institute of Basic Medical Sciences, Academy of
Military Medical Sciences, Beijing 100850, China )
Abstract Wortmannin,
a specific inhibitor of PI-3 kinase, antagonized the inhibitory effect of IL-6
in M1 acute myeloid leukemia cells. Electrophoretic mobility shift assays
revealed that wortmannin selectively reduced the IL-6-induced activation of
Stat3, while Stat3 plays a central role in IL-6-induced growth-arrest and
terminal differentiation of M1 cells. These results indicated that PI-3 kinase
was involved in the signal transduction of IL-6 and contributed to the
activation of Stat3.
Key words PI-3 kinase； wortmannin； IL-6； Stat3
*Corresponding author： Tel, 86-10-66931325； Fax, 86-10-68159436； e-mail： [email protected]