Deinococcus radiodurans
CatB Gene： Cloning and
Expression in Escherichia coli
ZHENG Hui1, Lü Xing*, XING Rui-yun, LU
Ping, YAO Qi-zhi1
( Beijing Institute of Radiation Medicine, Beijing 100850, China； 1Graduate School, University of Science
and Technology of China, Beijing 100039, China )
Abstract A 1 611 bp
length complete coding sequence of the catalase (Cat) was obtained through
bioimformatical analysis of the database of D.radiodurans genome, and
then was amplified from D. radiodurans genomic DNA by polymerase chain
reaction. The amplified gene was cloned into pKK223-3 vector and transformed
into E.coli UM2, a Cat-deficient mutant. Staining of non-denaturing
polyacrylamide gels for Cat activity demonstrated that pKK223-3 insert encoded
a Cat that co-migrated with the CatB found in D.radiodurans cell
lysates. Expression of CatB gene from D.radiodurans in E.coli
UM2 restored the resistance to H2O2 at low
concentrations.
Key words Deinococcus radiodurans； catalase； ionizing radiation
*Corresponding author： Tel, 86-10-66932210； Fax, 86-10-68214653； e-mail, [email protected]