Molecular Design and
Bio-activity Analysis of a t-PA Derivative (t-PA S165W)
ZHOU Hong1,2 WANG Guo-Li2, HUANG
Pei-Tang2 , HUANG Cui-Fen2*
( 1Burn Institute, Southwest Hospital, Third Military Medical
University, Chongqing 400038, China； 2Institute of Biotechnology, Academy of
Military Medical Sciences, Beijing 100071, China )
Abstract In order to
obtain tissue-type plasminogen activator (t-PA) mutant with enhanced affinity
for fibrin, redesigning t-PA strategies were proposed by computer molecular
designing technology, and the re-designed t-PA derivative, t-PA S165W, was
expressed in CHO cells. The bio-activities and affinity for fibrin of the
expression product were tested. No apparent changes were observed between t-PA
S165W and wild type t-PA. Therefore, single site mutation of the t-PA could not
lead to enhanced affinity for fibrin of t-PA.
Key words molecular designing technology； t-PA S165W； affinity for fibrin
*Corresponding author： Tel, 86-10-66948801； Fax, 86-10-63815186； e-mail： [email protected]