Active cis-elements of
PCNA Promoter in HeLa, HepG2, L-02 and MCF-7 Cell Lines
WANG Lei, LI Ming, YAN Pei-Jun, LU Chang-De*
( Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai
200031, China )
Abstract Proliferating
cell nuclear antigen (PCNA) is an auxiliary factor of DNA polymerase δ and ε
and functions in DNA replication and repair. Using PCR methods, 17 sites within
the human PCNA promoter from ＋60 to －538 were subjected to a 8 bp
substitution mutagenesis. Wild type promoter and each mutated promoters were
inserted into luciferase expression vector pGL2-Basic. These nonmutated and
mutated PCNA promoters were assayed by transient transfection of the plasmids
into HeLa, HepG2, L-02 and MCF-7 cell line, respectively. It was found that
several sites were common cis acting elements and several sites were
cell-specific cis acting elements. Data were further presented using in
vitro transcription assay with HeLa and HepG2 nuclear extract.
Key words proliferating cell nuclear antigen
(PCNA)； cis-elements； substitute-mutation； in vitro transcription
*Corresponding author： Tel, 86-21-64374430-234； Fax： 86-21-64338357； e-mail： [email protected]