Identification of the
Peptides that Bind to Tyrosine Kinase Receptor EphB2 by Phage Display
ZHANG Xiao-Guang, YAO Li-Bo*, HAN Jiong, LIU
Xin-Ping, HAN Jing-Tian1, NIE Xiao-Yan, SU Cheng-ZHi
( Department of Biochemistry and Molecular Biology, the Fourth Military
Medical University, Xi’an 710032, China； 1Department of Micorobiology, Medical
College of Chinese People’s Armed Police Forces, Tianjin 300162, China )
Abstract The gene
encoding the Ig-like domain of tyrosine protein kinase receptor EphB2 was
cloned into the expressing vector pET28a. Under induction with IPTG, the
positive strain expressed the fusion protein with a hexahistidine tail on the
N-terminal. The protein was purified under denaturing conditions using metal
chelate chromatography. The purity was up to 94%. The purified-protein-coated
ELISA plate was used as target to screen recombinant phages able to bind onto
it, and after three rounds of affinity screening, 19 phages that could bind
specifically with EphB2 were isolated from a random phage-displayed
seven-peptide library. The peptide sequences of the positive phage clones were
analyzed.
Key words EphB2； expression； metal chelate chromatography； phage display； random peptide library
*Corresponding author： Tel, 86-29-3374513； e-mail, [email protected]