Arginine
Vasopressin(4-8) Mobilizes Intracellular Calcium in C6 Glioma Cells
DONG Ming, XU Kan-Yan, ZHEN Xiao-Guang, DU Yu-Cang*
( Shanghai Institute of Biochemistry, Shanghai Institutes for Biological
Sciences, the Chinese Academy of Sciences, Shanghai 200031, China
)
Abstract To
understand the mechanism of neurotrophic action of neuropeptide ZNC(C)PR, which
could affect growth of C6 cells, fluorescent dye Fluo-3 and confocal laser
scanning microscope were used to assay the intracellular calcium in C6 glioma
cells. It was found that ZNC(C)PR and it’s analogue NLPR could mobilize
intracellular calcium in a dose-dependent manner. The ZNC(C)PR antagnist,
ZDC(C)PR, could inhibit the process, and the extracellular calcium did not
influence it.
Key words ZNC(C)PR；calcium；C6 glioma cells；fluo3；confocal laser scanning
microscope
*Corresponding author：Tel,86-21-64374430-277；Fax,86-21-64338357；e-mail,[email protected]