ZNC(C)PR Induces
Phosphorylation of CREB in Rat Hippocampus
DONG Ming, XIONG Ying, XU Kan-Yan, DU Yu-Cang*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract ZNC(C)PR
can facilitate the learning and memory in rat. Transgenic experiments have
revealed that long-term memory depended on cyclic AMP-response element binding
protein, CREB. CREB phosphorylation at serine-133 is essential for it’s
transcriptional activity. Here, it was demonstrated that ZNC(C)PR could induce
CREB phosphorylation at serine-133 in both rat hippocampus and rat hippocampus
slices. ZDC(C)PR［antagnist of ZNC(C)PR］, PTX(inhibitor of Go/Gi
protein coupled receptor), GF109203x(inhihitor of PKC), PD98059( inhibitor of
MAPK ) but not KN-62(inhibitor of CaMKII) could inhibit the phosphorylation of
CREB induced by ZNC(C)PR.
Key words ZNC(C)PR；hippocampus；CREB
*Corresponding author：Tel,021-64374430-277；Fax,021-64338357；e-mail,[email protected]