Cloning and Preliminary
Characterization of Regulatory Domain of Mouse Nestin Gene
CHENG Le-Ping, YANG Jing, JING Nai-He*
( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences,
Shanghai 200031, China )
Abstract Mouse
nestin genomic DNA(23.3 kb)was obtained after genomic library screening.
Sequence comparison of the cDNA and genomic DNA revealed three introns in this
gene. The positions and length of these introns are conserved among rat and
human. The effect of the second intron of mouse nestin gene on the expression
of luciferase reporter gene was investigated in vitro. Transient
transfection of the second intron and its series deletions of mouse nestin gene
showed that the second intron of mouse nestin gene could enhance reporter gene
expression and the activity was contributed by a fragment about 800 bp long of
its 3′end.
Key words mouse；nestin gene；gene structure；regulatory element
*Corresponding author：Tel,86-21-64374430；Fax,86-21-64338357；e-mail,[email protected]