Molecular Cloning of a
Gene Suppressed by Heat Shock and Its Expression Characteristics
DAI Jian-Xin*, WANG Yi-Lun, ZHOU Feng-Juan, LU
De-Ru
( Department of Molecular Genetics, Second Military Medical University,
Shanghai 200433, China )
LIU Yu-Jian, XU Ren-Bao
( Department of Pathophisiology, Second Military Medical University,
Shanghai 200433, China )
Abstract On the base
of a cDNA fragment cloned from a human osteosarcoma cell line (HOS-8603) which
was taken as the model of heat shock by DDRT-PCR, the cDNA library was screened
by the probe made from this fragment, and the colony containing full length
cDNA of this cDNA fragment (HSSG-1) was identified. Sequencing data
indicated that this cDNA consisted of 1 456 bp, coding for 276 amino acids.
Computer aided homology analysis did not find the same sequence published. The
result of RNA dot hybridization suggested that HSSG-1 was expressing
in many kinds of organs and cells, and the down regulating of its expression
level caused by heat shock was general. Therefore, this may be a gene that is
suppressed by heat shock.
Key words heat shock；gene expression；expression regulation；differential display；gene cloning
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