Binding of Human Cardiac
Myosin Light Chain 1 to Heavy Chain and Actin
HUANG Ren-Jian, PENG Bao-Zhen, ZHOU Guo-Ying, SHEN Ju-Ying,
GONG Zu-Xun
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for
Biological Sciences, Chinese Academy of Sciences, Shanghai 200031,
China )
Abstract
The cloning and sequence analysis of Chinese human cardiac myosin light chain 1
(CCMLC1) was previously reported. In this paper the cDNA of CCMLC1 was used as
template and both of cDNAs of N and C terminal fragments of CCMLC1, each
containing 98 amino acid residues, were obtained by PCR. Using the expressed
products of both fragments, the binding experiments of two fragments to
cardiacmyosin heavy chain of rat, human cardiac actin and to monoclonal
antibody raised against CCMLC1, have been performed, respectively, by means of
precipitation with GST Sepharose beads. The results showed that all the heavy
chain, actin and monoclonal antibody bound the N terminal fragment of CCMLC1 at
different sites. Under experimental conditions, the binding of CCMLC1 with
actin could affect the subsequent binding of CCMLC1 to heavy chain in
topologically.
Key words binding；human cardiac myosin light
chain 1；
cardiac myosin heavy chain； actin； monoclonal antibody
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