Barotolerant E. coli Induced by
High Hydrostatic Pressure
GAO Xiang, LI Jiong, RUAN Kang-Cheng*
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for
Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031,
China )
Abstract The
barotolerant E.coli TG1P, DH5αP, HB101P were obtained from TG1,DH5α,
HB101 after treatment with high hydrostatic pressure, and the survival rates of
the formers raised 2–3 magnitude, from 1.06×10-6, 2.98×10-4,
3.65×10-3 to 8.31×10-3, 3.40×10-1, 1.69×10-1,
respectively. Comparison of partial proteomes of wild and barotolerant bacteria
of TG1 and DH5α with two-dimensional electrophoresis showed that three proteins
were obviously highly expressed in barotolerant bacteria. The N terminal
sequence and molecular weight of one protein was shown to be V(L)EAGEFFMRA and
21 kD, with high identity to a known outmembrane protein in E.coli.
Key words high hydrostatic pressure；barotolerant Escherichia
coli；proteomes
*Corresponding author： Tel, 86-21-64740532；Fax, 86-21-64338357；
e-mail, [email protected]