SCAIF80, a Novel
Inhibitor of Angiogenesis, and Its Effect on Tumor Growth
SHEN Xian-Rong1,2, JI Dong-Mei1,HU
You-Qing1,JIA Fu-Xing2, WANG Ling2,
CHU Zhi-Yong2,REN Da-Ming１*
( 1State Key Laboratory of Genetic Engineering, Institute of
Genetics, Fudan University, Shanghai 200433, China；2Naval Medical Research
Institute；Shanghai 200433, China )
Abstract A novel
inhibitor of angiogenesis named SCAIF80 (shark cartilage-derived angiogenesis
inhibitory factor) from shark cartilage has been isolated and characterized.
SDS-PAGE analysis followed by silver staining revealed a single band with
molecular weight (Mr) of 80 kD. To determine whether this
protein was capable of inhibiting angiogensis, it was assayed in endothelial
cell (EC) proliferation and migration assay. The results showed that SCAIF80
significantly suppressed EC proliferation and migration in a dose-dependent
manner. In the chick chorioallantoic membrane (CAM) assay, SCAIF80 also showed
a potent inhibitory activity on angiogenesis in vivo. In animal tests,
the growth of tumor was potently suppressed by SCAIF80 therapy. Lewis lung
carcinoma was inhibited by 93.83 % at a dose of 5 mg/(kg・d). These findings
suggest that shark cartilage may produce a novel protein with anti-angiogenic
and anti-tumor activity.
Key words SCAIF80；angiogenesis；shark cartilage；CAM；endothelial cells
*Corresponding author：Tel,86-21-65642506；Fax,86-21-65511778；e-mail,[email protected]