Treatment of Parkinson’s
Disease by Genetically Modified Immortalized Fibroblasts
XU De-Hua*,CHEN Xian-Wen1,XU
Ling-Fei,CAO Lei,CHEN Sheng-Di1,LIU Xin-Yuan,ZHENG Zhong-Cheng
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for
Biological Sciences,the Chinese Academy of Sciences, Shanghai 200031,
China；1Shanghai Second Medical University, Rui
Jin Hospital,Shanghai 200035,China
)
Abstract Primary rat
fibroblast cells were immortalized by genetic modification of SV40 large T
antigen (LTAg) gene and called RFLT. This cell line was
non-tumorigenic after grafting into nud-mouse and rat. The LTAg
gene stopped expression when the cells were transplanted in rat striatum, but
it resumed the expression ability after the transplanted cells were recovered
from striatum and cultured in vitro.TH gene and GCH
gene were transfected into RFLT, respectively, and two types of transfected
cells, RFLT-TH and RFLT-GCH, were obtained. In mixed culture with these two
cell lines, DA was detected with HPLC-ECD. Implanting mixture of those cells
into the striatum of PD rats significantly decreased their rotational asymmetry
for up to 12 weeks. The expression of TH gene was proved by TH immunohistochemical
staining in the sections of rat brain. The establishment of the genetically
modified immortalized cells may play role in the gene therapy of PD.
Key words Parkinson’s disease；fibroblasts；SV40LT antigen gene；TH gene
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