A Vaccine to
Coxsackievirus Prepared by High Pressure
CHEN Zhong-Ming, TIAN Shao-Min, RUAN Kang-Cheng*
( Institute of Biochemistry and Cell Biology, Shanghai Institutes for
Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031,
China )
Abstract Effects of
high hydrostatic pressure on infectious and immune activity of coxsackievirus
group B (CVB) was described. CVB was completely inactivated at 230 MPa pressure
and -16 ℃. The inactivated viruses remained highly immunogenic and induced
CVB-specific serum antibody at a concentration as low as 1∶1 500. 67% mice
vaccinated with the pressurized viruses survived the challenge of fatal dose of
CVB. These results indicated that the pressurized CVB induced immune reactions
to protect mice from the viruses, and would be a potential vaccine candidate
for human myocarditis.
Key words high hydrostatic pressure；coxsackievirus group B (CVB)；immunogenicity；vaccine
*Corresponding author：Tel,86-21-64740532；Fax,86-21-64338357；e-mail,[email protected]