Structural Analysis of a
Gene Cluster Encoding Two Cationic and Three Anionic Peroxidases from Rice
Chromosome 4
CHEN Ze-Hua, ZHOU Bo, HAN Bin, QIAN Yue-Min, HONG Guo-Fan*
( National Center for Gene Research, Chinese Academy of Sciences, Shanghai 200233,
China )
Abstract Sequence
analysis of a rice BAC q3037(H0207F01) identified a cluster of five tandemly
arranged peroxidase genes, osp1,osp2,osp3,osp4
and osp5, within a 22.5 kb region. osp4,osp5 each
have three exons interrupted by two introns, while osp1,osp2
and osp3 each have two exons interrupted by a single intron. The five
genes were predicted products of 338, 335, 336, 343 and 346 amino acid
residues, respectively, including putative signal peptide sequence at the
amino-termini. And OSP1, OSP4 and OSP5 were predicted to be anionic peroxidase,
OSP2 and OSP3 are cationic. Comparative analysis and evolutionary analysis of
the clustered genes and other peroxidase family members revealed that the gene
cluster occurred by tandemly gene duplications(from osp5 to osp1)；and that osp5,ap1
and prx7 were potential orthologies, and osp1-5,ap1
and prx7 constituted a novel evolutionary branch of class III
peroxidases.
Key words peroxidase； gene cluster； tandemly gene duplication； ortholog
*Corresponding author： Tel, 86-21-64516371； Fax, 86-21-64825775； e-mail, [email protected]