Genome Structure and the
p10 Gene of the Helicoverpa
armigera Nucleopolyhedrovirus*
ZHANG Chuan-Xi*, WU Jia-Cai
( Institute of Applied Entomology, Zhejiang University, Hangzhou 310029,
China )
Abstract The genomic
DNA of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus
(C1 clone) was digested with BamHI, EcoRV, HindIII, KpnI,
PstI and XbaI, respectively, and formed 11, 31, 13, 6, 7, and
25 fragments larger than 400 bp, respectively. The size of genome was estimated
to be about 130.7 kb. A detailed physical map was constructed for the six
restriction enzymes. The five homologous region, hr1, hr2, hr3, hr4 and hr5,
and the ten genes including polyhedrin gene (ph), immediate-early
gene(ie1), p74, p10, chitinase gene, DNA
directed DNA polymerase gene (DNApol), helicase gene, superoxide
dismutase gene(sod), alkaline-exonuclease gene (alk-exo),
ecdysteroid UDP-glucosyltransferase gene (egt) were identified and
their locations in the genome were determined. The genome organization of
HaSNPV is quite different from those of other NPVs ever determined. The p10
gene was located in the fragment BamHI-I(1.89 kb) with the
transcriptional direction opposite to the polyhedrin gene. Upstream and
downstream of the p10 gene were p26 and p74 gene,
respectively. The transcriptional direction of p26 is the same as that
of p10 gene, and opposite to that of the p74 gene. The ORF
encoding p10 was 261 nucleotide long and encoding a putative 87 amino
acid polypeptide of 9.3 kD. The immediate upsteam region of the p10
was an A-rich region, and aconserved TAAG motif, associated with
transcriptional start sites in other p10 genes, was identified at a
site 52 nucleotides upstream of the start codon ATG. A putative polyadenylation
signal, AATAAA, was found 20 nucleotides downstream of the termination codon.
Key words HaSNPV； Physical map； genome organization； homologous region； nucleotide sequence of p10
*Corresponding author： Tel, 86-571-6971697； Fax, 86-571-6049815； e-mail, [email protected]